This assignment involves you designing some PCR primers that would help you clone a cDNA
of your gene of interest into the pUC18 vector.
❖ Once you have completed all the above steps, each group will submit a sheet which
includes the following information:
1. The Name of cDNA/mRNA, or the protein it encodes.
2. The Accession number of your mRNA/cDNA sequence (from the GenBank entry)
3. The name of the two restriction enzymes that you will using in your cloning method and their
restriction sites.
4. The two primer sequences including restriction enzyme sites.
5. Melting temperature calculations for both primers
◦ make sure you include the restriction site nucleotides in the calculations
6. Include an appendix part at the end that shows screenshots the steps that you have followed
to answer questions 3, 4 and 5.
Note: when you’re trying to show the binding of your primers to the template DNA, you need
to change the font used to display DNA sequences to “Courier” or “Courier New”.
use the uploaded file below for any further information + ask me for anything thanks.
