Antibiotic resistant discovery using soil sample and lab techniques to identify microorganisms that are in the soil. This experiment produce more knowledge about antibiotics resistance. Soil was collecting and was tested to determine the identity or a high close contact to the microbes that live there. This essay is a summary of the experiments that were performed to determine the identification.
used : agar plates. chose 2 eskape pathogens which were Escherichia coil and Myrobacterium.
used: the website blast to identify the sequence of DNA found in sample of soil.
Experiment 1: Devise a method to transfer microbes from a soil sample to a medium in the lab..
Experiment 2: Find a local soil environment you wish to sample..
Experiment 3: Find a method to isolate single colonies of bacteria from your soil sample.
Experiment 4: Choose your own media and culture conditions.
Experiment 5: Isolate unique colonies to test for antibiotic production.
Experiment 6: Understand the significance of the ESKAPE pathogens and using safe relatives in the lab.
Experiment 7: Design a method to screen for antibiotic producers.
Experiment 8: Initial identification of antibiotic-producing isolate.
Experiment 9: Testing isolate’s organic extract for antibiotic activity
Experiment 10: Testing isolate’s resistance to common antibiotics
Experiment 11: Biochemical characterization of isolates
Experiment 12: Assess activity against eukaryotes, potential use as biological control, and ecological relationships with other organisms..
outline :
This research has been conducted for the Brackenridge Park. Antibiotics have begun to become ineffective due to bacterial resistance. In order to combat this antibiotic resistance, soil cultures were taken from Brackenridge Park sources to begin screening for bacterial isolates that could be antibiotic producing. The location of choice was San Antonio’s Brackenridge Park. Due to the surrounding of the park there will be an abundance of diversity of microbes in the studied soil sample which will lead to the production of antibiotics in the soil.This experiment was performed to determine if any isolates produce antibiotics near the San Antonio Breckenridge park. Currently, there is a limited amount of antibiotics and the number of new antibiotics discovered is decreasing. If antibiotic-producing microorganisms are discovered, this can assist the issue by providing a new antibiotic to be tested.