Analysis of next-generation sequencing data specifically of staphylococcus aureus bacteria for a master’s thesis. Use of the Galaxy (bioinformatics online tool) tool for analysis of genomes and identification of genes for resistance, infectivity, etc.
I want you to help me write the bibliography / theory, which will certainly include the evolution (historical review) of sequencing technologies, how the development of new sequencing technologies (NGS) has helped the progress of microbiology, what are the conventional methods of identifying microbes and what the advantages of NGS, a few words about Illumina, and then an analysis of staphylococcus aureus, why it is an important pathogen, how much resistance it has developed to which antibiotics, etc.
Bacterial genome terminology (contigs, scaffolds, coverage, depth of sequencing etc) A few words about galaxy and what tools we used for fastqc, fastp, assembly and identification of MRA and limogenicity genes. Setting up the pipeline (I can send you exactly the tutorials that the professor suggested I follow to set it up) Finally, if there are recent Staphylococcus aureus Genome Analysis Studies (especially with Galaxy) and more, let’s mention her and tell her what do we get Analysis of tangible galaxy results and conclusions